tag:blogger.com,1999:blog-6334453475526523597.post1771429729851329689..comments2023-11-07T10:31:25.370+00:00Comments on CoreGenomics: London Calling: nanopores updatedJames@cancerhttp://www.blogger.com/profile/02825715598810395734noreply@blogger.comBlogger3125tag:blogger.com,1999:blog-6334453475526523597.post-11778412021826810112016-11-03T06:28:49.000+00:002016-11-03T06:28:49.000+00:00Some really cool pics....but proof of pudding is i...Some really cool pics....but proof of pudding is in the eatin. SmidgION looks cool..but the processing power & storage space to run & store nanopore data is huge. Unless it's connected to a laptop computer/transmits data to a cloud faster than current limits. Or Oxford Nanopore comes up with the next gen super smartphone I wouldn't see it coming up soon. Voltrax looks like a digital microfluidic device which mixes two reagents metered & pippeted onto it. Anyone familiar with digital microfluidics technology should look up what is already done using this technology. Also look up Illumina automated sample prep using NeoPrep. Anonymousnoreply@blogger.comtag:blogger.com,1999:blog-6334453475526523597.post-47241766200550539402016-07-01T12:48:50.119+01:002016-07-01T12:48:50.119+01:00Also it would be nice if they publish some real us...Also it would be nice if they publish some real users feed back (not just their best runs) - with respect of reliability, optimal library sizes (Loading efficiency does decrease with insert size, so going over 40Kb would require new loading approaches), actual systematic errors profiles - there are areas in reads which have 98% accuracy followed by the 85%, followed by completely random sequence (30-500bp stretches). <br />Pore blocking still is a big issue with R9 - from ~100-120 stranded pores in the first 3 hours it can come down to ~50 stranded pores after 18 hours. Some approach for unblocking is needed (like piezzo element, which can shear off DNA blob).<br />Also they do not mention the requirement for degassing of the fuel mix/nuclease free water in their kits - otherwise one sees a lot of tiny bubbles forming on the ASIC array.Anonymousnoreply@blogger.comtag:blogger.com,1999:blog-6334453475526523597.post-64457885916377483072016-06-01T11:51:03.588+01:002016-06-01T11:51:03.588+01:00Thanks for the nice summary!
Just a comment on th...Thanks for the nice summary! <br />Just a comment on the cost figures for the PromethION: $2-3/Gb and $600-2.5k/cell - this does not match. I think the costs/Gb are their vision once crumpet chips are there. The $600-2.5k/cell would translate to $20-100/Gb (assuming 20% efficiency and 48h life time). That is more in the price range of HiSeq or MiSeq/Sequel.Anonymoushttps://www.blogger.com/profile/01383011243619877738noreply@blogger.com