tag:blogger.com,1999:blog-6334453475526523597.post2911599773905185954..comments2023-11-07T10:31:25.370+00:00Comments on CoreGenomics: MRSA testing by NGSJames@cancerhttp://www.blogger.com/profile/02825715598810395734noreply@blogger.comBlogger1125tag:blogger.com,1999:blog-6334453475526523597.post-29812640290378884202012-12-07T19:33:22.367+00:002012-12-07T19:33:22.367+00:00Hi James. Culture is very cheap, a few quid. You c...Hi James. Culture is very cheap, a few quid. You can do about 15-25 MRSA genomes on a v1 MiSeq run (2Mb genome x 30-fold coverage, allowing for some variation in coverage. So that's between £30 and £50 for the sequencing per isolate. The rest will be library prep, again around £50 for barcoded Nextera. How do you get Nextera down to a fiver a throw? We would love to do that!<br />Nick Lomanhttps://www.blogger.com/profile/12121179953421841062noreply@blogger.com