tag:blogger.com,1999:blog-6334453475526523597.post3507573501847023070..comments2023-11-07T10:31:25.370+00:00Comments on CoreGenomics: How old can a PCR machine be whilst still being useful?James@cancerhttp://www.blogger.com/profile/02825715598810395734noreply@blogger.comBlogger1125tag:blogger.com,1999:blog-6334453475526523597.post-7070241202439379972012-10-04T11:41:49.076+01:002012-10-04T11:41:49.076+01:00I used to use a waterbath PCR machine back when I ...I used to use a waterbath PCR machine back when I used to to alellic discrimination by FRET (TaqMan). 150 x 384 well plates in one go.<br />We'd even do one plate at time in them as the waterbath method is actually far superior to a thermal block. The ramp times between denature/anneal/extend are only couple of seconds so it left less opportunity for strange things to happen.Anonymousnoreply@blogger.com