tag:blogger.com,1999:blog-6334453475526523597.post6605644039505892415..comments2023-11-07T10:31:25.370+00:00Comments on CoreGenomics: Nanopore sir? should be delivered in 2014 or perhaps 2030!James@cancerhttp://www.blogger.com/profile/02825715598810395734noreply@blogger.comBlogger1125tag:blogger.com,1999:blog-6334453475526523597.post-30129568746826863492013-11-23T06:04:52.016+00:002013-11-23T06:04:52.016+00:00I am so tired of both academic and commercial enti...I am so tired of both academic and commercial entities overstating the value of their widget and underplaying the competition. Nanopores will never be a "sample in-data out" deal. Careful attention to temperature, salt concetration, sample purity, etc will be required. In that the light, ONT's addition of a motor, hairpin, and insertion adapter via a transposon are inconveniences on par with Illumina's Nextera sample prep, which everyone seems to love so much. I have always felt that ONT's Achilles heel is the lipid bilayer and the limited scale-up headroom. There are many ways to EVENTUALLY overcome these in a synthetic pore. It will still be 2020 at least for that though, not because we will be drilling holes sequentially in a TEM (only an academic with an army of slave labor - aka grad students - can contemplate that but because it will take that long for the technology to develop the precision required.Anonymousnoreply@blogger.com