tag:blogger.com,1999:blog-6334453475526523597.post7958030864790771651..comments2023-11-07T10:31:25.370+00:00Comments on CoreGenomics: MiSeq: possible growth potential part 2James@cancerhttp://www.blogger.com/profile/02825715598810395734noreply@blogger.comBlogger3125tag:blogger.com,1999:blog-6334453475526523597.post-36484644660527639422011-11-22T16:21:17.558+00:002011-11-22T16:21:17.558+00:00Shawn: I hope HiSeq is not dead yet! There are lik...Shawn: I hope HiSeq is not dead yet! There are likely to be improvements but we have seen such a ramp up in data volumes from the instrument since its release that I guess we should not get too greedy. If some of the quality improvments can be ported over from MiSeq then PE150 and 1TB becomes possible outside of Illumina labs.<br />Can't you shed any light on this?<br /><br />djschlesinger: You would sarifice numbers of reads but would get higher physical coverage of the genome of interest. 1kb inserts is essentialy mate-pair without the mate-pair protocol. Yes clusters would be larger and less intense and this may impact %PF rates as well. Something to think about though.James@cancerhttps://www.blogger.com/profile/02825715598810395734noreply@blogger.comtag:blogger.com,1999:blog-6334453475526523597.post-57993598748900513762011-11-21T21:45:16.222+00:002011-11-21T21:45:16.222+00:00Any thought as to how insert length affects cluste...Any thought as to how insert length affects cluster formation? I would imagine at some point (perhaps >1kb) clusters would become difficult to discern. I guess you could always decrease your density on the flowcell, but then you're sacrificing output.Anonymousnoreply@blogger.comtag:blogger.com,1999:blog-6334453475526523597.post-37386353682415482062011-11-18T17:43:26.934+00:002011-11-18T17:43:26.934+00:00James, assuming that it will be possible to get 35...James, assuming that it will be possible to get 35Gb out of a MiSeq run, it will take some development time to get to that point. Are you getting the sense from Illumina that during that time there won't be any more improvements on the HiSeq platform?Anonymoushttps://www.blogger.com/profile/08590569202982138251noreply@blogger.com