tag:blogger.com,1999:blog-6334453475526523597.post3993545778846538886..comments2023-11-07T10:31:25.370+00:00Comments on CoreGenomics: MiSeq: possible growth potential part 4James@cancerhttp://www.blogger.com/profile/02825715598810395734noreply@blogger.comBlogger2125tag:blogger.com,1999:blog-6334453475526523597.post-39158189099604958382013-01-21T11:55:04.670+00:002013-01-21T11:55:04.670+00:00Hi James, I was wondering if you could comment on ...Hi James, I was wondering if you could comment on the issues that led to your MiSeq being replaced? We have had a lot of problems with our MiSeq - new hard drive, three new reagent pumps, new mirror... <br /><br />I met you a few years ago in Glasgow at an Illumina meeting but if you could email me I'd really appreciate it! Gavin dot Wilkie at glasgow dot ac dot ukAnonymousnoreply@blogger.comtag:blogger.com,1999:blog-6334453475526523597.post-36345145960744042902012-11-19T13:13:38.671+00:002012-11-19T13:13:38.671+00:00We're finding that the increased read length h...We're finding that the increased read length has killed 454 and will most likely kill Ion Torrent. The only think stopping it is the diversity issue. Lots of people are wanting to sequence single (or a few) amplicons at really high depth across a number of patients/samples. For example to look at heterogeneity of tumor samples, or for 16S work. The PhiX spike-in is a messy work-around - who wants to lose 50% of their run to sequence a phage that has been sequenced to a astronomical depth already?!Unknownhttps://www.blogger.com/profile/14869462307678967101noreply@blogger.com