tag:blogger.com,1999:blog-6334453475526523597.post6063877304677675372..comments2023-11-07T10:31:25.370+00:00Comments on CoreGenomics: My (almost) patent removing the need for users to quantitate before clusteringJames@cancerhttp://www.blogger.com/profile/02825715598810395734noreply@blogger.comBlogger2125tag:blogger.com,1999:blog-6334453475526523597.post-79724692632210687692012-09-17T22:34:36.736+01:002012-09-17T22:34:36.736+01:00Yes I think this will be added to every TruSeq kit...Yes I think this will be added to every TruSeq kit over the next year. However it means throwing most of your library down the sink. The method suggested by me (or Illumina in their patent) potentially allows very low input libraries to be added to the flowcell with no amplification or quantification and that would be pretty cool!James@cancerhttps://www.blogger.com/profile/02825715598810395734noreply@blogger.comtag:blogger.com,1999:blog-6334453475526523597.post-14785777493851556852012-09-13T23:27:16.853+01:002012-09-13T23:27:16.853+01:00Perhaps they're going to stick with the Nexter...Perhaps they're going to stick with the NexteraXT-type system of library normalisation using binding to limited concentrations magnetic beads. It's not as elegant a system, and it may not work as well in terms of cluster spacing (c.f. cluster number - although I'm not sure either system would do that much differently), but it's certainly easier for Illumina to implement since it doesn't require any flowcell manufacturing changes... just a few extra tubes in the box. Anonymoushttps://www.blogger.com/profile/03176782496993845014noreply@blogger.com