tag:blogger.com,1999:blog-6334453475526523597.post8467214019263925444..comments2023-11-07T10:31:25.370+00:00Comments on CoreGenomics: How good is your NGS multiplexing?James@cancerhttp://www.blogger.com/profile/02825715598810395734noreply@blogger.comBlogger3125tag:blogger.com,1999:blog-6334453475526523597.post-9630328671886273812013-10-22T07:12:54.142+01:002013-10-22T07:12:54.142+01:00Have anyone tried using the SequalPrepâ„¢
Normalizat...Have anyone tried using the SequalPrepâ„¢<br />Normalization Plate for Amplicon (Invitrogen-Life Tech) on these type of libraries? It can bind approx 25ng per well. Or perhaps using the same principle with Illumina primers covering the wells of the normalization plate in a custom assay?<br />Anonymousnoreply@blogger.comtag:blogger.com,1999:blog-6334453475526523597.post-24647581086785625402013-10-20T23:22:00.417+01:002013-10-20T23:22:00.417+01:00This size/clustering problem is even important for...This size/clustering problem is even important for the simpler case of pooling lots of Nextera reactions that had high variance in final fragment size distribution. My labmates and I have been thinking about this issue a lot. We really need a model that can go from size distribution in to size distribution out.Taminoreply@blogger.comtag:blogger.com,1999:blog-6334453475526523597.post-51098933073736325022013-10-18T20:54:58.735+01:002013-10-18T20:54:58.735+01:00So do you lose some reads to issues with barcodes?...So do you lose some reads to issues with barcodes? For example an error in the barcode read or the barcode missing?<br /><br />Also you don't have issues with cross contamination or carry over contamination from run to run?Anonymoushttps://www.blogger.com/profile/04344987411651421193noreply@blogger.com