I’ll start off by saying not quite, but you can read on to get an idea on how to increase read length of a MiSeq 500cycle v2 kit to get 600bp of data.
How to hack your MiSeq kit: Make sure you follow the instructions on adding a little extra reagent to some of the wells.
5 mL of incorporation buffer from well 1 of a left-over reagent cartridge to well 1 of the 2x301 cartridge.
7 mL of scan mix from well 2 of a left-over reagent cartridge to well 2 of the 2x301 cartridge.
6.8 mL of cleavage mix from well 4 of a left-over reagent cartridge to well 4 of the 2x301 cartridge.
80 mL of incorporation buffer from a left-over incorporation buffer bottle.
Now simply set the Miseq to 2x301 in the samplesheet and ignore the warning the software gives. Et voila 600bp for the price of 500. With a MiSeq v3 kit costing about $1400 that’s potentially a $400 saving.
Will we be doing this in my lab? No way, I’m far too conservative with users samples to play around like this. But I wish I could do more stuff like this, as it’s fun. It makes me want to come up with my own genomics Instructables.
Watch out for their paper: Saunders, A.M., Albertsen, M., McIllroy, S.J. & Nielsen, P.H. (in prep) MiDAS: the field guide to the activated sludge ecosystem.